دانلود کتاب Bacteriophage T4 tail fibers as a basis for structured assemblies

فهرست مطالب :

Content: 1. Introduction --
2. Tail fiber function and structure --
2.1 Hypothesis --
2.2 Staged or ordered assembly --
2.3 Possible applications --
3. Tail fiber production and purification --
4. Tail fiber modifications --
4.1 Deletions and insertions --
4.2 Coiled-coil assembly segment modifications --
4.2.1 Biological role of the gp 37 coiled-coil --
4.2.2 Coiled-coils as intrinsic chaperones --
4.3 Inserts conferring novel functionality --
4.3.1 Insertion and testing of an antibody binding epitope --
4.3.2 Insertion and testing of a biotinylation site --
4.3.3 Attaching magnetic nanoparticles via a biotin linkage --
4.3.4 Potential tail fiber-nanoparticle system improvements --
5. Conclusions --
Appendices --
A. Tail fiber purification --
B. Preparation of head-tail (HT) complexes --
C. Purified tail fiber assay --
D. Analytical ultracentrifugation --
E. Electron microscopy --
F. Bacteria and bacteriophage strains --
G. Media and buffers --
H. Phage stock growth and construction of phage with multiple mutations --
I. Transfer of engineered mutations into phage --
J. PCR and sequencing primers --
K. Adsorption rate measurements --
L. Construction of extended coiled-coil region --
M. Mab binding assays --
N. Construction of biotinylated phage --
O. Western blot and SDS-PAGE analysis --
P. Purification of biotinylated tail fibers --
Q. Preparation of nanoparticles --
R. Attachment of nanoparticles to biotinylated tail fibers --
S. Dynamic light scattering (DLS) --
T. AC magnetic susceptometry theory --
U. Magnetic susceptibility measurements --
About the authors --
References --
Index.