دانلود کتاب Cancer Metabolism: Methods and Protocols

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Front Matter ....Pages i-xi
Metabolic Labeling of Cultured Mammalian Cells for Stable Isotope-Resolved Metabolomics: Practical Aspects of Tissue Culture and Sample Extraction (Daniel R. Crooks, Teresa W.-M. Fan, W. Marston Linehan)....Pages 1-27
Imaging Cancer Metabolism with Positron Emission Tomography (PET) (Timothy H. Witney, David Y. Lewis)....Pages 29-44
Radioluminescence Microscopy: A Quantitative Method for Radioisotopic Imaging of Metabolic Fluxes in Living Cancer Cells (Debanti Sengupta, Guillem Pratx)....Pages 45-53
Use of 13C315N1-Serine or 13C515N1-Methionine for Studying Methylation Dynamics in Cancer Cell Metabolism and Epigenetics (Alice C. Newman, Christiaan F. Labuschagne, Karen H. Vousden, Oliver D. K. Maddocks)....Pages 55-67
Measurement of Mitochondrial Membrane Potential with the Fluorescent Dye Tetramethylrhodamine Methyl Ester (TMRM) (Sarah Creed, Matthew McKenzie)....Pages 69-76
Assessment of Stabilization and Activity of the HIFs Important for Hypoxia-Induced Signalling in Cancer Cells (David Kung-Chun Chiu, Misty Shuo Zhang, Aki Pui-Wah Tse, Carmen Chak-Lui Wong)....Pages 77-99
Determination of Polarization of Resident Macrophages and Their Effect on the Tumor Microenvironment (Ioannis S. Pateras, Tomer Cooks)....Pages 101-112
Quantitation of Macropinocytosis in Cancer Cells (Koen M. O. Galenkamp, Basheer Alas, Cosimo Commisso)....Pages 113-123
Integrated Analysis of Acetyl-CoA and Histone Modification via Mass Spectrometry to Investigate Metabolically Driven Acetylation (Simone Sidoli, Sophie Trefely, Benjamin A. Garcia, Alessandro Carrer)....Pages 125-147
Methods to Measure Autophagy in Cancer Metabolism (Natalia von Muhlinen)....Pages 149-173
Lipidomic Analysis of Cancer Cell and Tumor Tissues (Islam Sk Ramiz, Soumen Kanti Manna)....Pages 175-204
Mass Spectrometry-Based Profiling of Metabolites in Human Biofluids (Tanushree Chakraborty, Soumen Kanti Manna)....Pages 205-234
Gas Chromatography-Mass Spectrometry and Analysis of the Serum Metabolomic Profile Through Extraction and Derivatization of Polar Metabolites (Jodi Rattner, Farshad Farshidfar, Oliver F. Bathe)....Pages 235-249
Metabolite Profiling of Clinical Cancer Biofluid Samples by NMR Spectroscopy (Beata Mickiewicz, M. Eric Hyndman, Hans J. Vogel)....Pages 251-274
Assessment of Metabolic Signature for Cancer Diagnosis Using Desorption Electrospray Ionization Mass Spectrometric Imaging (Shibdas Banerjee, Soumen Kanti Manna)....Pages 275-297
Compositional Analysis of the Human Microbiome in Cancer Research (Elisa Morales, Jun Chen, K. Leigh Greathouse)....Pages 299-335
Assessing Metabolic Dysregulation in Muscle During Cachexia (Myriam Y. Hsu, Paolo E. Porporato, Elisabeth Wyart)....Pages 337-352
Using Seahorse Machine to Measure OCR and ECAR in Cancer Cells (Jing Zhang, Qing Zhang)....Pages 353-363
Metabolic Profiling of Live Cancer Tissues Using NAD(P)H Fluorescence Lifetime Imaging (Thomas S. Blacker, Michael D. E. Sewell, Gyorgy Szabadkai, Michael R. Duchen)....Pages 365-387
Overview of Characterizing Cancer Glycans with Lectin-Based Analytical Methods (Amanda J. Pearson, Elyssia S. Gallagher)....Pages 389-408
Hyperpolarized MRI for Studying Tumor Metabolism (Mikko I. Kettunen)....Pages 409-426
Overview of Glutamine Dependency and Metabolic Rescue Protocols (Shuo Qie, Dan He, Nianli Sang)....Pages 427-439
Integration of Metabolomics and Transcriptomics to Identify Gene-Metabolite Relationships Specific to Phenotype (Andrew Patt, Jalal Siddiqui, Bofei Zhang, Ewy Mathé)....Pages 441-468
Biclustering Analysis of Co-regulation Patterns in Nuclear-Encoded Mitochondrial Genes and Metabolic Pathways (Robert B. Bentham, Kevin Bryson, Gyorgy Szabadkai)....Pages 469-478
Using the Human Genome-Scale Metabolic Model Recon 2 for Steady-State Flux Analysis of Cancer Cell Metabolism (Lake-Ee Quek, Nigel Turner)....Pages 479-489
Back Matter ....Pages 491-495