توضیحاتی در مورد کتاب :
هورمون های استروئیدی ترکیبات منحصر به فردی هستند که در فصل مشترک رویدادهای غدد درون ریز محیطی و مکانیسم های عصبی فعال هستند. بنابراین اثرات آنها یک سیستم سیگنالینگ محیطی مهم را برای تغییر عملکرد مغز ارائه می دهد. این جلد تکنیکهای پیشرفته و کلاسیک را برای مطالعه هورمونهای استروئیدی و گیرندههای آنها و اثرات و اعمال آنها ارائه میکند.
ویژگی های کلیدی
پروتکل های جامع برای مطالعه
* سینتیک و متابولیسم استروئید
* گیرنده های استروئید
* اثرات مولکولی و سلولی استروئیدها
* اثرات استروئیدی بر روی یکپارچه سیستم های
فهرست مطالب :
Content:
Methods in Neurosciences
Page ii
Front Matter
Page iii
Copyright page
Page iv
Contributors to Volume 22
Pages ix-xiii
Preface
Page xv
E. RONALD DE KLOET, WIN SUTANTO
Methods in Neurosciences
Pages xvii-xviii
1 - Steroid Hormone Transport through Blood–Brain Barrier: Methods and Concepts
Pages 3-22
William M. Pardridge
2 - Enhanced Delivery of Steroids to the Brain Using a Redox-Based Chemical Delivery System
Pages 23-35
James W. Simpkins, Nicholas Bodor
3 - Neurosteroids: Biosynthesis and Function
Pages 36-50
Paul Robel, Etienne-Emile Baulieu
4 - Cytochrome P450 Enzymes in Brain
Pages 51-66
Margaret Warner, Adrian Wyss, Shigetaka Yoshida, Jan-Åke Gustafsson
5 - Methods for Estimating 11β-Hydroxysteroid Dehydrogenase Activity
Pages 67-76
Zygmunt Krozowski
6 - Steroid Hormone Binding to Intracellular Receptors: In Vitro and in Vivo Studies
Pages 79-95
Aernout D. van Haarst, Thomas F. Szuran
7 - Steroid Hormone Binding to Membrane Receptors
Pages 96-115
Miles Orchinik, Thomas F. Murray
8 - In Vitro Autoradiography for Steroid Receptors
Pages 116-142
Neil J. MacLusky, He Yuan, Debbie Bowlby, Richard B. Hochberg, Theodore J. Brown
9 - Immunocytochemical Studies on Glucocorticoid Receptor
Pages 143-161
Antonio Cintra, Gunnar Akner, Rafael Coveñas, Mercedes de León, Ann-Charlotte Wikström, Luigi F. Agnati, Jan-Åke Gustafsson, Kjell Fuxe
10 - Confocal Scanning Laser Microscopy of Steroid Receptors in Brain
Pages 162-174
Bas van Steensel, Erica P. van Binnendijk, Roel van Driel
11 - Ultrastructural Aspects of Steroid Receptor Localization: Immunocytochemical Perspective
Pages 175-188
Zsolt Liposits, Imre Kalló
12 - Hybridization Studies of Adrenocorticosteroid Receptors in the Central Nervous System
Pages 189-210
James P. Herman, Seung P. Kwak, Stanley J. Watson
13 - Detection and Characterization of Epalon Receptors: Novel Recognition Sites for Neuroactive Steroids That Modulate the GABAA Receptor Complex
Pages 211-225
Linda D. McCauley, Kelvin W. Gee
14 - Mutation Analysis of Steroid Hormone Receptors
Pages 226-241
Michael Karl, Heinrich M. Schulte, George P. Chrousos
15 - Protein–DNA-Binding Assay for Analysis of Steroid-Sensitive Neurons in Mammalian Brain
Pages 245-264
Yuan-shan Zhu, Donald W. Pfaff
16 - Gene Transfection Studies Using Recombinant Steroid Receptors
Pages 265-276
Klaus Damm
17 - Regulation of Neuropeptide Genes: Determination of Responsiveness to Steroids and Identification of Receptors in Brain Nuclei
Pages 277-295
Joke J. Cox, Sofia Lopes da Silva, Wiljan Hendriks, J. Peter H. Burbach
18 - Cloning of Steroid-Responsive mRNAs by Differential Hybridization
Pages 296-313
Nancy R. Nichols, Jeffrey N. Masters, Caleb E. Finch
19 - Molecular Correlates of Corticosterone Action in Hippocampal Subregions
Pages 314-329
Suresh M. Nair, James H. Eberwine
20 - Glucocorticoid-Determined Protein Synthesis
Pages 330-341
Linda A. Dokas
21 - Use of Antisense Oligodeoxynucleotides to Block Gene Expression in Central Nervous System
Pages 342-356
Margaret M. McCarthy
22 - Steroid Regulation of Neuronotrophic Activity: Primary Microcultures of Midbrain Raphe and Hippocampus
Pages 359-371
Efrain C. Azmitia, Xiao Ping Hou
23 - Central Glucocorticoid Receptors and Neuronal Plasticity
Pages 372-382
Kjell Fuxe, Antonio Cintra, Gerson Chadi, Jan-Åke Gustafsson, Luigi F. Agnati
24 - Steroid Action on Neuronal Structure
Pages 383-402
Catherine S. Woolley, Elizabeth Gould
25 - Electron Microscopic Double and Triple Labeling Immunocytochemistry in Elucidation of Synaptological Interactions between Ovarian Steroid-Sensitive Neurons and Circuits
Pages 403-416,416a,417-434
Csaba Leranth, Frederick Naftolin, Marya Shanabrough, Tamas L. Horvath
26 - Gene-Mediated Steroid Control of Neuronal Activity
Pages 435-445
Marian Joëls
27 - Electrophysiological Studies of Neurosteroid Modulation of γ-Aminobutyric Acid Type A Receptor
Pages 446-467
C. Hill-Venning, D. Belelli, J.A. Peters, J.J. Lambert
28 - In Vitro Approaches to Studying Glucocorticoid Effects on Gene Expression in Neurons and Glia
Pages 468-480
Martha Churchill Bohn
29 - Behavioral Approaches to Study Function of Corticosteroids in Brain
Pages 483-495
Melly Silvana Oitzl
30 - Adrenocorticosteroids and Cardiovascular Regulation: Methods for Surgery and Blood Pressure Measurements
Pages 496-509
Elise P. Gómez Sánchez
31 - Steroids and Central Regulation of Immune Response
Pages 510-522
Bernd Schöbitz
32 - Steroid Hormone Effects on Brain: Novel Insights Connecting Cellular and Molecular Features of Brain Cells to Behavior
Pages 525-542
Bruce S. McEwen
Index
Pages 543-552
توضیحاتی در مورد کتاب به زبان اصلی :
Steroid hormones are unique compounds in that they are active at the interface of peripheral endocrine events and neural mechanisms. Thus their effects present an important peripheral signaling system to alter brain function. This volume presents state-of-the-art and classical techniques for the study of steroid hormones and their receptors and their effects and actions.
Key Features
Comprehensive protocols included for the study of
* Steroid kinetics and metabolism
* Steroid receptors
* Molecular and cellular effects of steroids
* Steroid effects on integrated systems