دانلود کتاب RNA Chaperones: Methods and Protocols

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Front Matter ....Pages i-xi
Biochemical Methods for the Study of the FinO Family of Bacterial RNA Chaperones (Hyeong Jin Kim, Steven Chaulk, David Arthur, Ross A. Edwards, J. N. Mark Glover)....Pages 1-18
Quantitative Analysis of RNA Chaperone Activity by Native Gel Electrophoresis and Fluorescence Spectroscopy (Subrata Panja, Ewelina M. Małecka, Andrew Santiago-Frangos, Sarah A. Woodson)....Pages 19-39
Fluorescent Molecular Beacons Mimicking RNA Secondary Structures to Study RNA Chaperone Activity (Pilar Menendez-Gil, Carlos J. Caballero, Cristina Solano, Alejandro Toledo-Arana)....Pages 41-58
Specific Nucleic Acid Chaperone Activity of HIV-1 Nucleocapsid Protein Deduced from Hairpin Unfolding (Micah J. McCauley, Ioulia Rouzina, Mark C. Williams)....Pages 59-88
Real-Time Fluorescence-Based Approaches to Disentangle Mechanisms of a Protein’s RNA Chaperone Activity (Tobias Schmidt, Susann Friedrich, Ralph P. Golbik, Sven-Erik Behrens)....Pages 89-106
Use of tRNA-Mediated Suppression to Assess RNA Chaperone Function (Jennifer Porat, Mark A. Bayfield)....Pages 107-120
Salt-Dependent Modulation of the RNA Chaperone Activity of RNA-Binding Protein La (Gunhild Sommer, Christina Sendlmeier, Tilman Heise)....Pages 121-136
Kinetic and Thermodynamic Analyses of RNA–Protein Interactions (Ryo Amano, Taiichi Sakamoto)....Pages 137-150
Detection of MicroRNAs Released from Argonautes (Kyung-Won Min, J. Grayson Evans, Erick C. Won, Je-Hyun Yoon)....Pages 151-159
Mapping the RNA Chaperone Activity of the T. brucei Editosome Using SHAPE Chemical Probing (W.-Matthias Leeder, H. Ulrich Göringer)....Pages 161-178
RNA Remodeling by RNA Chaperones Monitored by RNA Structure Probing (Susann Friedrich, Tobias Schmidt, Sven-Erik Behrens)....Pages 179-192
In Vivo RNA Chemical Footprinting Analysis in Archaea (Robert Knüppel, Martin Fenk, Michael Jüttner, Sébastien Ferreira-Cerca)....Pages 193-208
RNA Structure Analysis by Chemical Probing with DMS and CMCT (José M. Andrade, Ricardo F. dos Santos, Cecília M. Arraiano)....Pages 209-223
Disordered RNA-Binding Region Prediction with DisoRDPbind (Christopher J. Oldfield, Zhenling Peng, Lukasz Kurgan)....Pages 225-239
Delivering Molecular Beacons via an Electroporation-Based Approach Enables Live-Cell Imaging of Single RNA Transcripts and Genomic Loci (Shiqi Mao, Yachen Ying, Xiaotian Wu, Antony K. Chen)....Pages 241-252
Site-Specific Dual-Color Labeling of Long RNAs (Meng Zhao, Richard Börner, Roland K. O. Sigel, Eva Freisinger)....Pages 253-270
Single-Molecule FRET Assay for Studying Cotranscriptional RNA Folding (Heesoo Uhm, Sungchul Hohng)....Pages 271-282
Characterizing Complex Nucleic Acid Interactions of LINE1 ORF1p by Single Molecule Force Spectroscopy (M. Nabuan Naufer, Mark C. Williams)....Pages 283-297
Isolation and Analysis of Bacterial Ribosomes Through Sucrose Gradient Ultracentrifugation (Ricardo F. dos Santos, Cecília M. Arraiano, José M. Andrade)....Pages 299-310
Back Matter ....Pages 311-314