دانلود کتاب A Laboratory Guide to In Vitro Studies of Protein-DNA Interactions

فهرست مطالب :

Front Matter....Pages 1-14
Introduction....Pages 15-17
The Study of Protein-DNA Interactions by Deoxyribonuclease I Footprinting....Pages 19-34
Exonuclease III Protection Assay for Specific DNA-Binding Proteins....Pages 35-43
The Proteolytic Clipping Band-Shift Assay of Protein-DNA Complexes....Pages 45-54
Visualising Intimate Protein-DNA Contacts and Altered DNA Structures with Ultravioltet Light....Pages 55-70
UV Cross-Linking of Protein to Bromouridine-Substituted RNA....Pages 71-78
Molecular Weight Determination of DNA-Binding Proteins by UV Cross-linking Combined with SDS Polyacrylamide Gel Electrophoresis....Pages 79-91
Interaction of Proteins with Partially Depurinated and Depyrimidinated Oligonucleotides (Missing-Contact Probing of Protein-DNA Interactions)....Pages 93-109
The Study of Protein-DNA Contacts by Ethylation Interference....Pages 111-120
The Study of Protein-DNA Interactions by Methylation Interference....Pages 121-131
Using the Chemistry of the Hydroxyl Radical to Determine Structural Details about DNA and Protein-DNA Complexes....Pages 133-144
Detection of Unusual DNA Structures and DNA-Protein Interactions by Diethylpyrocarbonate Carbethoxylation....Pages 145-152
Electron Microscope Visualisation of Protein-DNA Complexes....Pages 153-161
Qualitative and Quantitative Studies of Protein-DNA Interactions by Gel Mobility-Shift Assay....Pages 163-194
Diagonal Gel Mobility-Shift Assays for the Resolution of Multi-Subunit Complexes Binding to Regulatory Elements of Specific Genes....Pages 195-206
A Rapid Gel-Shift Technique to Analyse DNA-Protein Interactions using Phastsystem™....Pages 207-219
Purification of Sequence-Specific DNA-Binding Proteins by Affinity Chromatography....Pages 221-231
Cloning of Sequence-Specific DNA-Binding Proteins by Screening λ cDNA Expression Libraries with Radiolabelled Binding-Site Probes....Pages 233-244
Detection of DNA Bending by Gel Electrophoresis: Use of Plasmid Vectors....Pages 245-257
The Use of Amplification for in vitro Footprinting Experiments....Pages 259-276
Non-radioactive Detection Methods for in Vitro Footprinting....Pages 277-290
Quantitative Determination of Proteins in Crude Extracts....Pages 291-298
Back Matter....Pages 299-328