دانلود کتاب A Laboratory Guide to the Mammalian Embryo

فهرست مطالب :

1. Follicle Development in Vitro......Page 12
2. Preparation of Gametes, in Vitro Maturation, in Vitro Fertilization, andEmbryo Recovery and Transfer......Page 33
3. Culture of the Mammalian Preimplantation Embryo......Page 50
4. Assessment Preimplantation Embryo Development and Viability......Page 71
5. Micromanipul of Gametes......Page 86
6.Micromanipul: Biopsy......Page 108
7. Analysis of Intracellular Ions in Embryos: pH and Calcium......Page 134
8. Assessment of Nutrient Uptake, Metabolite Production, and EnzymeActivity......Page 148
9. Metabolic Pathway Activity......Page 163
10. Confocal Imaging of Structural Molecules in Mammalian Gametes......Page 174
11. FISH for Detection of Aneuploidy in SingleHuman Blastomeres for Preimplantation Genetic Diagnosis......Page 193
12. Assessment of Ploidy, Telomere Length, and Telomerase Activityin Oocytes and Embryos......Page 202
13. Microscale RNA Isolation from Mammalian Embryos......Page 226
14. Relative mRNA Transcript Abundance in Early Embryos by ReverseTranscription-PCR......Page 234
15.Characterization of Novel Genes during Early Development by Applicationof Differential Display RT–PCR......Page 246
16.Two-Dimensional Protein Gel Database Analysis of Embryos, Oocytes,and Oocyte-Associated Granulosa Cells......Page 256
17. Antisense Disruption of Gene Expression in the PreimplantationEmbryo......Page 267
18. Gene Subtraction and Analysis......Page 279
19. Analysis of Apoptosis in the Preimplantation Embryo......Page 288
20. Cytoplasmic Signaling and Cell Cycle Control in the Mouse Eggand Embryo......Page 307
21. Human Embryonic Stem Cells......Page 322
22.Spermatogonial Transplant......Page 343
23. Somatic Cell Nuclear Transfer......Page 361
24.Cryobiology: Slow Freezing Vitrification of Embryos......Page 384
Index......Page 400