دانلود کتاب Advanced Microscopy in Mycology

فهرست مطالب :

Preface......Page 6
Contents......Page 9
Contributors......Page 10
1.1 Introduction......Page 12
1.2 The Confocal Microscopy System......Page 13
1.3 Lasers......Page 17
1.5 Assessing Image Quality and the Performance of the Confocal System......Page 18
1.6 Pros and Cons of Confocal Microscopy......Page 21
1.8 Comparison of LSCM with Spinning-disc, Deconvolution and Two-Photon Methods......Page 22
1.9 Fungal Sample Preparation......Page 24
1.10 Image Processing and Movie-making......Page 28
1.11.3 How to Assess the Fitness of Living Cells?......Page 29
1.11.6 How to Deal with Bleed-Through in Multi-labeled Specimens?......Page 30
1.12 Conclusion......Page 31
References......Page 32
2.1 Introduction......Page 37
2.2.1 Bimolecular Fluorescence Complementation (BiFC)......Page 39
2.2.2 Föster Resonance Energy Transfer (FRET)......Page 41
2.2.3 Fluorescence Lifetime Imaging Microscopy (FLIM) and FLIM–FRET......Page 42
2.3 Protein Localization and Dynamics......Page 43
2.3.1 Fluorescent Recovery After Photobleaching (FRAP)......Page 44
2.3.2 Photoconvertible Tagging of Filamentous Fungal Proteins......Page 46
2.4.1 The Photoconvertible Fluorescent Protein mEosFP......Page 48
2.5 Conclusions and Future Perspectives......Page 49
References......Page 51
3.1 Introduction......Page 57
3.2 Structured Illumination Microscopy......Page 59
3.2.1 Applications in Mycological Imaging......Page 61
3.3 Stimulated Emission Depletion Microscopy......Page 62
3.3.2 Stimulated Emission Depletion Microscopy Summary......Page 64
3.4 Localization Microscopy......Page 65
3.4.1 Applications in Mycological Imaging......Page 66
3.4.2 LM Summary......Page 67
3.5 Summary......Page 68
References......Page 69
4.1 Introduction......Page 71
4.2.1 FTIR Spectroscopy......Page 72
4.2.2 Classification Methods......Page 73
4.2.4 Sample Preparation......Page 74
4.2.5 FTIR Mapping......Page 76
4.2.7 Synchrotron FTIR Microscopy......Page 77
4.2.8 Micro- and Macro-ATR Analysis......Page 78
4.3 Chemical Imaging of Fungi......Page 79
4.3.1 Single Hyphae......Page 80
4.3.2 Monolayers of Single Cells, Mycelium Pellets and Fruiting Bodies......Page 83
4.4.1 Spectroscopic Techniques......Page 85
4.4.2 Microscopic Techniques......Page 87
4.5 Biotic Interactions......Page 88
4.5.1 Wood Degradation......Page 89
4.5.2 Detection of Fungal Infestation and Mycotoxins......Page 92
4.6 Conclusions and Outlook......Page 93
References......Page 94
5.1 Introduction......Page 99
5.2.1 Light Source......Page 103
5.2.3 X-ray Microscope Objective Lens......Page 104
5.3.1 Introduction to Tomography......Page 106
5.3.2 Tomographic Data Collection and Processing......Page 107
5.4 Cell Culture and Preparation......Page 108
5.5 Cell Organelles Identified......Page 109
5.6 Quantitative Analysis of Organelles in the Cell......Page 111
5.7 Future Developments of Hard X-ray Microscopy......Page 112
References......Page 114
6.1 In Situ Nanocharacterization System Inside the ESEM......Page 118
6.2.1 Nanoneedle Fabrication by FIB......Page 121
6.2.2 Mathematical Model to Determine Stiffness......Page 122
6.2.3 Proof of Principle: Cell Stiffness Measurement......Page 123
6.3.2 Mathematic Model for Adhesion Force Measurements......Page 124
6.4.1 Schematics of the Experimental Setup......Page 126
6.5 Yeast Cell Cutting: Towards Next Generation of Nanosurgery......Page 127
6.5.1 Preparation of the Nanoknife......Page 128
6.5.2 Single-Cell Slicing with the Nanoknife......Page 129
6.6 Conclusions and Future Prospects......Page 130
References......Page 131
7.1 Introduction......Page 133
7.2 Immobilization of Yeast Cells......Page 135
7.3 Imaging Living Yeast Cells by Atomic Force Microscopy......Page 136
7.4 Probing the Nanomechanical Properties of Yeast Cells......Page 139
7.5 Single-Cell and Molecule Force Spectroscopy......Page 141
References......Page 146
8.1 Introduction......Page 150
8.2 Advances in the Application of Biosensors......Page 151
8.3 Three-Dimensional Electron Microscopy......Page 152
8.4 Correlative Microscopy......Page 154
8.5 New Imaging Technologies......Page 160
8.6 Conclusion......Page 164
References......Page 165
Index......Page 170