دانلود کتاب Chromatography Principles and Instrumentation

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Cover
Title Page
Copyright
Contents
Preface
Chapter 1 Fundamentals of Chromatography
1.1 Theory
1.1.1 Component Separation
1.1.2 Retention Factor
1.1.3 Separation
1.1.4 Resolution and Theoretical Plates
1.2 Band Broadening
1.2.1 Diffusion
1.2.2 Linear Velocity
1.2.3 Broadening in Open Tubes with No Stationary Phase and No Retention
1.2.4 Broadening in Open Tubes with a Stationary Phase
1.2.5 Broadening in a Packed Column
1.2.6 Putting It All Together
1.2.7 Practical Consequences of Broadening Theory
1.3 General Resolution Equation
1.4 Peak Symmetry
1.5 Key Operating Variables
1.6 Instrumentation
1.7 Practice of The Technique
1.7.1 Quantitation
1.7.2 Internal Standards and the Method of Standard Additions
1.8 Emerging Trends and Applications
1.9 Summary
Problems
References
Further Reading
Chapter 2 Gas Chromatography
2.1 Theory of Gas Chromatographic Separations
2.1.1 GC Columns and Partitioning
2.2 Key Operating Variables that Control Retention
2.2.1 Adjusting Retention Time: Temperature
2.2.2 Adjusting Retention Time: Temperature Programming
2.2.3 Adjusting Retention Time: Mobile Phase Flow Rate
2.2.4 Adjusting Retention Time: The Column and the Stationary Phase
2.2.5 Adjusting Retention Time: Summary
2.2.6 Measures of Retention
2.3 Gas Chromatography Instrumentation
2.3.1 Carrier Gas Supply
2.3.2 The Injection Port and the Solute Injection Process
2.3.3 Oven/Column Compartment
2.3.4 Detectors
2.4 A More Detailed Look at Stationary Phase Chemistry: Kovats Indices and Mcreynolds Constants
2.4.1 Kovats Retention Indices
2.4.2 Stationary Phase Selection
2.5 Gas Chromatography in Practice
2.5.1 Syringe Washing
2.5.2 Controls and Blanks/Ghost Peaks
2.5.3 Autosamplers
2.5.4 GC Septa
2.5.5 Qualitative Analysis
2.5.6 Quantitative Analysis
2.5.7 Derivatization
2.5.8 High-Speed GC
2.5.9 Tandem GC
2.5.10 Microfabricated GC
2.6 A \"Real-World\" Application of Gas Chromatography
2.6.1 GC and International Oil Trading
2.7 Summary
Problems
References
Further Reading
Chapter 3 Liquid Chromatography
3.1 Examples of Liquid Chromatography Analyses
3.2 Scope of Liquid Chromatography
3.3 History of LC
3.3.1 Modern Packing Materials
3.4 Modes of Liquid Chromatography
3.4.1 Normal Phase Liquid Chromatography (NPLC)
3.4.2 Reversed-Phase Liquid Chromatography (RPLC)
3.4.3 Ion-Exchange Chromatography (IEX)
3.4.4 Hydrophilic Interaction Chromatography (HILIC)
3.4.5 Size Exclusion Chromatography (SEC)
3.4.6 Affinity Chromatography
3.5 HPLC Instrumentation
3.5.1 The Proportioning Valve
3.5.2 Mixing Chamber
3.5.3 Pumps
3.5.4 Injection
3.5.5 The Column and Particles
3.5.6 Guard Columns
3.5.7 Detectors
3.6 Specific Uses of and Advances in Liquid Chromatography
3.6.1 Chiral Separations
3.6.2 Preparative-Scale Chromatography
3.6.3 Ultra-High Performance Liquid Chromatography (UHPLC) for High-Speed Separations
3.6.4 Tandem-Column Liquid Chromatography
3.6.5 Two-Dimensional Liquid Chromatography (2D-LC)
3.7 Application of LC - Analysis of Pharmaceutical Compounds in Groundwater
3.7.1 Sampling
3.7.2 Analysis Method for 21 Antibiotics - Sample Pretreatment
3.7.3 Use of Internal Standards and Other Quality Assurance Issues
3.7.4 LC Analyses
3.7.5 Mass Spectrometric Selected Ion Monitoring Detection
3.7.6 Results
3.8 Summary
Problems
References
Solutions
Index
EULA