دانلود کتاب Decellularization Methods of Tissue and Whole Organ in Tissue Engineering

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Introduction
Readership
Contents
About the Editor
Affiliations and Expertise
References
1 Characterization Methods of Acellularized Tissue and Organs
Abstract
1.1 History
1.1.1 Hematoxylin–Eosin Staining (H & E)
1.1.2 4′,6-Diamidino-2-Phenylindole (DAPI)
1.1.3 The MTT Cell Proliferation Assay
1.1.4 Electron Microscopy
1.1.5 Second Harmonic Generation
1.1.6 Mechanical Properties
1.1.7 Zymography
1.2 Conclusion
References
2 Esophagus Decellularization
Abstract
2.1 History
2.2 Material and Methods
2.2.1 Animal Models
2.2.2 Decellularization
2.2.3 Sterilization
2.2.4 Characterization of the Decellularized Matrix
2.2.5 DNA Quantification
2.2.6 General Structure and Composition
2.2.7 Biomechanical Properties
2.2.8 Immunogenicity and Biocompatibility
2.2.9 Cytotoxicity
2.3 Cell Seeding
2.3.1 Cell Types and Origin
2.3.2 Seeding Methods
2.4 Clinical Applications
References
3 Fetal Lung Tissue Engineering
Abstract
3.1 History
3.2 Lung Tissue Engineering
3.3 Material and Methods
3.4 Scaffold Preparation
3.4.1 In Rat Lung Model
3.4.2 In Human and Porcine Model
3.4.3 In Rabbit Model
3.5 Casting and SEM of Casts
3.6 SEM
3.7 Mechanical Tests
3.8 DNA Staining and Quantification
3.9 Collagen
3.10 Sulfated Glycosaminoglycans
3.11 Elastin
3.12 Cell Culture and Seeding
3.13 Bioreactor
3.14 Histological Evaluation
3.14.1 Histomorphological Evaluation
3.15 Radiography, MRI, and CT Scan
3.16 Limitation
3.17 Conclusion
References
4 Lung Extracellular Matrix as a Platform for Lung Organ Bioengineering: Design and Development of Tissue Engineered Lung
Abstract
4.1 History
4.2 Materials and Methods
4.2.1 Decellularization Technology
4.3 Tests to Confirm the Efficacy of the Decellularization Process
4.4 Cell Seeding
4.5 Clinical Applications
4.6 Limitations
4.7 Conclusion
References
5 Cardiac Extracellular Matrix as a Platform for Heart Organ Bioengineering: Design and Development of Tissue-Engineered Heart
Abstract
5.1 History
5.2 Materials and Methods
5.3 Cell Seeding
5.4 Clinical Applications
5.5 Limitations
5.6 Conclusion
References
6 The Most Ideal Pancreas Extracellular Matrix as a Platform for Pancreas Bioengineering: Decellularization/Recellularization Protocols
Abstract
6.1 History
6.2 Materials and Methods
6.3 Decellularization Technology
6.4 Whole Pancreas Decellularization and Regenerative Medicine
6.5 Tests to Confirm the Efficacy of the Decellularization Process
6.6 Cell Seeding
6.7 Clinical Applications
6.8 Limitations
6.9 Conclusion
References
7 Decellularization of Small Intestinal Submucosa
Abstract
7.1 History
7.2 Materials and Methods
7.3 Cell Seeding
7.4 Clinical Applications
7.5 Limitations
7.6 Conclusion
References
8 The Isolated Mucosa of the Rat Colon Decellularization, Microscopy and Cell Cultures
Abstract
8.1 History
8.2 Material and Methods
8.2.1 Animal Care
8.3 Organ Dissection and Tissue Preparation
8.4 Decellularization
8.5 Light and Electron Microscopy of Decellularized Colon Mucosa
8.6 Cell Cultures
8.7 Discussion
8.8 Conclusions
Acknowldgements
References
9 The Renal Extracellular Matrix as a Supportive Scaffold for Kidney Tissue Engineering: Progress and Future Considerations
Abstract
9.1 History
9.2 Materials and Methods
9.3 Cell Seeding
9.4 Clinical Applications
9.5 Limitations
9.6 Conclusion
References
10 Production and Preparation of Porcine Urinary Bladder Matrix (UBM) for Urinary Bladder Tissue-Engineering Purposes
Abstract
10.1 History
10.2 Materials and Methods
10.2.1 ECM Tissue Harvest and Preparation
10.2.2 UBM Sheet Manufacture
10.3 Cell Seeding
10.3.1 Cell Culture
10.3.2 Maintenance of Culture
10.3.3 Subcultures
10.3.4 Cell-Seeding Techniques
10.3.5 AlamarBlue™ Experimental Protocol to Assess Cell Viability on UBM
10.3.6 Fluorescence
10.4 Clinical Applications
10.5 Limitations
10.6 Conclusion
References
11 Decellularization Methods of Ovary in Tissue Engineering
Abstract
11.1 History
11.2 Materials and Methods
11.3 Cell Seeding
11.4 Clinical Applications
11.5 Limitations
11.6 Conclusion
References
12 Decellularization Methods of Uterus in Tissue Engineering
Abstract
12.1 History
12.2 Materials and Methods
12.3 Cell Seeding
12.4 Clinical Applications
12.5 Limitations
12.6 Conclusion
References
13 Decellularization Methods of Vagina and Cervix in Tissue Engineering
Abstract
13.1 History
13.2 Materials and Methods
13.3 Cell Seeding
13.4 Clinical Applications
13.5 Limitations
13.6 Conclusions
References
14 Decellularization of Male Reproductive Tissue
Abstract
14.1 History
14.1.1 Decellularization of Testicular Tissue
14.2 Decellularization of Whole Testis
14.3 Conclusion
Acknowledgements
References
15 Decellularization of Skin Tissue
Abstract
15.1 History
15.2 Properties of an Ideal Biomaterial
15.3 Strategies for Developing Acellular Dermal Matrix from Different Skin Tissues
15.3.1 Decellularization
15.3.2 Acellular Matrices
15.4 Development of Acellular Dermal Matrices from Different Species of Animals
15.4.1 Histopathological Evaluation
15.4.2 DNA Quantification
15.4.3 Sodium Dodecyl Sulphate Polyacrylamide Gel Electrophoresis (SDS-PAGE)
15.5 Protocols for Preparing Acellular Dermal Matrices
15.5.1 Protocol-I (Trypsin–Sodium Deoxycholate Combination)
15.5.2 Protocol-II (Trypsin-Sodium Dodecyl Sulphate Combination)
15.5.3 Protocol-III (Trypsin-Tritron-×100 Combination)
15.5.4 Protocol-IV (Trypsin-Tween-20 Combination)
15.5.5 Optimization of Protocols for Rabbit Skin
15.5.5.1 Protocols for De-Epithelization
15.5.5.2 Protocols for Preparation of Acellular Dermal Matrix
15.5.5.3 Microscopic Observations
15.5.6 Optimization of Protocols for Pig Skin
15.5.6.1 Protocols for De-Epithelization
15.5.6.2 Protocols for Preparation of Acellular Dermal Matrix
15.5.6.3 Microscopic Observations
15.5.7 Optimization of Protocols for Goat Skin
15.5.7.1 Protocols for De-Epithelization
15.5.7.2 Protocols for Preparation of Acellular Dermal Matrix
15.5.7.3 Microscopic Observations
15.5.8 Optimization of Protocols for Sheep Skin
15.5.8.1 Protocols for De-Epithelization
15.5.8.2 Protocols for Preparation of Acellular Dermal Matrix
15.5.8.3 Microscopic Observations
15.5.9 Optimization of Protocols for Buffalo Skin
15.5.9.1 Protocols for De-Epithelization
15.5.9.2 Protocols for Preparation of Acellular Dermal Matrix
15.5.9.3 Microscopic Observations
15.5.10 Optimization of Protocols for Rat Skin
15.5.10.1 Protocols for De-Epithelization
15.5.10.2 Protocols for Preparation of Acellular Dermal Matrix
15.5.10.3 Microscopic Observations
15.5.11 Another Protocol for Preparation of Acellular Matrix from Rabbit Dermis
15.6 Skin Tissue Engineering
15.7 Skin Tissue Engineering Work Done in the Division of Surgery
15.8 Clinical Application of Developed Dermal Matrices in Different Species of Animals
15.8.1 Dermal Matrices Tested in Buffalo
15.8.2 Dermal Matrices Tested in Cattle
15.8.3 Dermal Matrices Tested in Goats
15.8.4 Dermal Matrices Tested in Horses
15.8.5 Dermal Matrices Tested in Dogs
15.9 Conclusion
References
16 Tissue Engineering in Skin Substitute
Abstract
16.1 History
16.2 Materials and Methods
16.2.1 Cell Seeding
16.2.2 Clinical Applications
16.2.3 Limitations
16.3 Conclusions
References
17 Extracellular Matrix Scaffold Using Decellularized Cartilage for Hyaline Cartilage Regeneration
Abstract
17.1 History
17.2 Materials
17.3 Methods
17.3.1 General Considerations
17.3.2 Tissue Harvesting
17.3.2.1 Decellularization
Decellularization Procedure
Characterization of the Decellularized Hyaline Cartilage
17.3.2.2 Recellularization
Isolation and Expansion of Chondrocytes
Isolation and Expansion of Mesenchymal Stem Cells
Cell Seeding
17.4 Research and Clinical Applications
17.5 Limitations
17.6 Conclusions
References
18 Decellularization of Bone Tissue
Abstract
18.1 History
18.2 Materials and Methods
18.3 Cell Seeding—Recellularization
18.4 Clinical Applications
18.5 Conclusion
References
19 Decellularization of Nervous Tissues and Clinical Application
Abstract
19.1 Peripheral Nervous System (PNS)
19.1.1 History
19.1.2 Materials and Methods
19.1.3 Clinical Applications of Decellularized Peripheral Nerves
19.1.4 Cell Seeding for Promotion of Peripheral Nerve Regeneration
19.1.5 Conclusions and Future Work on PNS Regeneration
19.2 Central Nervous System (CNS)
19.2.1 History
19.2.2 Materials and Methods
19.2.2.1 Decellularized Nerve Tissues for SCI Regeneration
19.2.2.2 Solubilized ECM for TBI Regeneration
19.2.3 Cell Transplantations for CNS Injuries
19.2.4 Conclusions and Future Works for CNS Regeneration
19.3 Overall Summary and Future Works
References
20 The Three-Dimensional Structure of Tissues and Organs: A Scanning Electron Microscopic Atlas for Research and Education
References