دانلود کتاب Disease Gene Identification: Methods and Protocols

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Front Matter ....Pages i-x
Front Matter ....Pages 1-1
Identification of Disease Susceptibility Alleles in the Next Generation Sequencing Era (Johanna K. DiStefano, Christopher B. Kingsley)....Pages 3-16
Induced Pluripotent Stem Cells in Disease Modeling and Gene Identification (Satish Kumar, John Blangero, Joanne E. Curran)....Pages 17-38
Development of Targeted Therapies Based on Gene Modification (Taylor M. Benson, Fatjon Leti, Johanna K. DiStefano)....Pages 39-51
What Can We Learn About Human Disease from the Nematode C. elegans? (Javier Apfeld, Scott Alper)....Pages 53-75
Microbiome Sequencing Methods for Studying Human Diseases (Rebecca M. Davidson, L. Elaine Epperson)....Pages 77-90
The Emerging Role of Long Noncoding RNAs in Human Disease (Johanna K. DiStefano)....Pages 91-110
Front Matter ....Pages 111-111
Identification of Disease-Related Genes Using a Genome-Wide Association Study Approach (Tobias Wohland, Dorit Schleinitz)....Pages 113-150
Whole Genome Library Construction for Next Generation Sequencing (Jonathan J. Keats, Lori Cuyugan, Jonathan Adkins, Winnie S. Liang)....Pages 151-161
Whole Exome Library Construction for Next Generation Sequencing (Winnie S. Liang, Kristi Stephenson, Jonathan Adkins, Austin Christofferson, Adrienne Helland, Lori Cuyugan et al.)....Pages 163-174
Optimized Methodology for the Generation of RNA-Sequencing Libraries from Low-Input Starting Material: Enabling Analysis of Specialized Cell Types and Clinical Samples (Kendra Walton, Brian P. O’Connor)....Pages 175-198
Using Fluidigm C1 to Generate Single-Cell Full-Length cDNA Libraries for mRNA Sequencing (Robert Durruthy-Durruthy, Manisha Ray)....Pages 199-221
MiSeq: A Next Generation Sequencing Platform for Genomic Analysis (Rupesh Kanchi Ravi, Kendra Walton, Mahdieh Khosroheidari)....Pages 223-232
Methods for CpG Methylation Array Profiling Via Bisulfite Conversion (Fatjon Leti, Lorida Llaci, Ivana Malenica, Johanna K. DiStefano)....Pages 233-254
Front Matter ....Pages 255-255
miRNA Quantification Method Using Quantitative Polymerase Chain Reaction in Conjunction with Cq Method (Fatjon Leti, Johanna K. DiStefano)....Pages 257-265
Primary Airway Epithelial Cell Gene Editing Using CRISPR-Cas9 (Jamie L. Everman, Cydney Rios, Max A. Seibold)....Pages 267-292
RNA Interference to Knock Down Gene Expression (Haiyong Han)....Pages 293-302
Using Luciferase Reporter Assays to Identify Functional Variants at Disease-Associated Loci (Anup K. Nair, Leslie J. Baier)....Pages 303-319
Front Matter ....Pages 321-321
Physiologic Interpretation of GWAS Signals for Type 2 Diabetes (Richard M. Watanabe)....Pages 323-351
Identification of Genes for Hereditary Hemochromatosis (Glenn S. Gerhard, Barbara V. Paynton, Johanna K. DiStefano)....Pages 353-365
Identification of Driver Mutations in Rare Cancers: The Role of SMARCA4 in Small Cell Carcinoma of the Ovary, Hypercalcemic Type (SCCOHT) (Jessica D. Lang, William P. D. Hendricks)....Pages 367-379
The Rise and Fall and Rise of Linkage Analysis as a Technique for Finding and Characterizing Inherited Influences on Disease Expression (Ettie M. Lipner, David A. Greenberg)....Pages 381-397
Back Matter ....Pages 399-400