دانلود کتاب Duchenne Muscular Dystrophy: Methods and Protocols

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Front Matter ....Pages i-xii
Front Matter ....Pages 1-1
An Overview of Recent Therapeutics Advances for Duchenne Muscular Dystrophy (Jean K. Mah)....Pages 3-17
Clinical Manifestations and Overall Management Strategies for Duchenne Muscular Dystrophy (Takeshi Tsuda)....Pages 19-28
Front Matter ....Pages 29-29
Cardiac Involvement in Duchenne Muscular Dystrophy and Related Dystrophinopathies (Sophie I. Mavrogeni, George Markousis-Mavrogenis, Antigoni Papavasiliou, George Papadopoulos, Genovefa Kolovou)....Pages 31-42
Characterization of the Inflammatory Response in Dystrophic Muscle Using Flow Cytometry (Jenna M. Kastenschmidt, Ileen Avetyan, S. Armando Villalta)....Pages 43-56
Imaging Analysis of the Neuromuscular Junction in Dystrophic Muscle (Stephen J. P. Pratt, Shama R. Iyer, Sameer B. Shah, Richard M. Lovering)....Pages 57-72
Front Matter ....Pages 73-73
System Biology Approach: Gene Network Analysis for Muscular Dystrophy (Federica Censi, Giovanni Calcagnini, Eugenio Mattei, Alessandro Giuliani)....Pages 75-89
Proteomic Profiling of the Dystrophin-Deficient Brain (Sandra Murphy, Kay Ohlendieck)....Pages 91-105
Probing the Pathogenesis of Duchenne Muscular Dystrophy Using Mouse Models (Alexander Morrison-Nozik, Saptarsi M. Haldar)....Pages 107-119
Front Matter ....Pages 121-121
Exon Skipping Therapy Using Phosphorodiamidate Morpholino Oligomers in the mdx52 Mouse Model of Duchenne Muscular Dystrophy (Shouta Miyatake, Yoshitaka Mizobe, Hotake Takizawa, Yuko Hara, Toshifumi Yokota, Shin’ichi Takeda et al.)....Pages 123-141
Designing Effective Antisense Oligonucleotides for Exon Skipping (Takenori Shimo, Rika Maruyama, Toshifumi Yokota)....Pages 143-155
Identification of Splicing Factors Involved in DMD Exon Skipping Events Using an In Vitro RNA Binding Assay (Julie Miro, Cyril F. Bourgeois, Mireille Claustres, Michel Koenig, Sylvie Tuffery-Giraud)....Pages 157-169
The Use of Antisense Oligonucleotides for the Treatment of Duchenne Muscular Dystrophy (Karima Relizani, Aurelie Goyenvalle)....Pages 171-183
PMO Delivery System Using Bubble Liposomes and Ultrasound Exposure for Duchenne Muscular Dystrophy Treatment (Yoichi Negishi, Yuko Ishii, Kei Nirasawa, Eri Sasaki, Yoko Endo-Takahashi, Ryo Suzuki et al.)....Pages 185-192
Front Matter ....Pages 193-193
Proton Nuclear Magnetic Resonance (1H NMR) Spectroscopy-Based Analysis of Lipid Components in Serum/Plasma of Patients with Duchenne Muscular Dystrophy (DMD) (Niraj Kumar Srivastava)....Pages 195-204
Test of Antifibrotic Drugs in a Cellular Model of Fibrosis Based on Muscle-Derived Fibroblasts from Duchenne Muscular Dystrophy Patients (Simona Zanotti, Marina Mora)....Pages 205-217
Flow Cytometry-Defined CD49d Expression in Circulating T-Lymphocytes Is a Biomarker for Disease Progression in Duchenne Muscular Dystrophy (Wilson Savino, Fernanda Pinto-Mariz, Vincent Mouly)....Pages 219-227
Front Matter ....Pages 229-229
Advanced Methods to Study the Cross Talk Between Fibro-Adipogenic Progenitors and Muscle Stem Cells (Luca Tucciarone, Usue Etxaniz, Martina Sandoná, Silvia Consalvi, Pier Lorenzo Puri, Valentina Saccone)....Pages 231-256
AAV6 Vector Production and Purification for Muscle Gene Therapy (Christine L. Halbert, James M. Allen, Jeffrey S. Chamberlain)....Pages 257-266
From gRNA Identification to the Restoration of Dystrophin Expression: A Dystrophin Gene Correction Strategy for Duchenne Muscular Dystrophy Mutations Using the CRISPR-Induced Deletion Method (Benjamin Duchêne, Jean-Paul Iyombe-Engembe, Joël Rousseau, Jacques P. Tremblay, Dominique L. Ouellet)....Pages 267-283
Back Matter ....Pages 285-287