دانلود کتاب Epitranscriptomics: Methods and Protocols

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Front Matter ....Pages i-xii
Bisulfite Sequencing of RNA for Transcriptome-Wide Detection of 5-Methylcytosine (Lukas Trixl, Dietmar Rieder, Thomas Amort, Alexandra Lusser)....Pages 1-21
Single-Molecule Analysis of RNA Dynamics in Living Cells Using Molecular Beacons (Mingming Chen, Shiqi Mao, Xiaotian Wu, Zhao Ma, Yantao Yang, Christopher J. Krueger et al.)....Pages 23-39
Visualization of Xist Long Noncoding RNA with a Fluorescent CRISPR/Cas9 System (Urszula Waśko, Zeming Zheng, Sanchita Bhatnagar)....Pages 41-50
HAMR: High-Throughput Annotation of Modified Ribonucleotides (Lee E. Vandivier, Zachary D. Anderson, Brian D. Gregory)....Pages 51-67
High-Resolution Mapping of N6-Methyladenosine Using m6A Crosslinking Immunoprecipitation Sequencing (m6A-CLIP-Seq) (Phillip J. Hsu, Chuan He)....Pages 69-79
Direct Chemical Biotinylation of RNA 5′-Ends Using a Diazo Reagent (Greggory M. Rice, Razvan Nutiu, Christian M. Gampe)....Pages 81-87
Identification of Methylated Transcripts Using the TRIBE Approach (Lina Worpenberg, Tobias Jakobi, Christoph Dieterich, Jean-Yves Roignant)....Pages 89-106
Decoding the Atlas of RNA Modifications from Epitranscriptome Sequencing Data (Xiao-Qin Zhang, Jian-Hua Yang)....Pages 107-124
Detection of MicroRNA-Mediated Target mRNA Cleavage and 3′-Uridylation in Human Cells by a SLA-RT-PCR Analysis (Jing Lin, Lin Ji)....Pages 125-136
Genome-Wide Annotation of circRNAs and Their Alternative Back-Splicing/Splicing with CIRCexplorer Pipeline (Rui Dong, Xu-Kai Ma, Ling-Ling Chen, Li Yang)....Pages 137-149
Synthesis and Evaluation of Novel Neamine–Nucleoside Conjugates as Potential Antibiotic Targets for Escherichia coli 16S Ribosomal RNA (Zhen-Jun Yang, Maria-Grazia Concilio, Vasudevan Ramesh, Li-He Zhang)....Pages 151-163
Electron Microscope Detection of 5-Methylcytosine on DNA and RNA (Irene Masiello, Marco Biggiogera)....Pages 165-177
Immuno-Northern Blotting: Detection of Modified RNA Using Gel Separation and Antibodies to Modified Nucleosides (Eikan Mishima, Takaaki Abe)....Pages 179-187
LncVar: Deciphering Genetic Variations Associated with Long Noncoding Genes (Xiaowei Chen, Yajing Hao, Ya Cui, Zhen Fan, Runsheng Chen)....Pages 189-198
Guided Reconstruction of Full-Length Isoforms from Short Reads by CIDANE (Sandro Andreotti, Stefan Canzar)....Pages 199-208
Profiling of N6-Methyladenosine in Zika Virus RNA and Host Cellular mRNA (Gianluigi Lichinchi, Tariq M. Rana)....Pages 209-218
Detection and Quantification of Pseudouridine in RNA (Hironori Adachi, Meemanage D. DeZoysa, Yi-Tao Yu)....Pages 219-235
5-Methylcytosine Analysis by RNA-BisSeq (Yu-Sheng Chen, Hai-Li Ma, Ying Yang, Wei-Yi Lai, Bao-Fa Sun, Yun-Gui Yang)....Pages 237-248
RNA Modification Regulatory Genes in DNA Damage (Radoslav Janostiak, Narendra Wajapeyee)....Pages 249-262
Dot Blot Analysis for Measuring Global N6-Methyladenosine Modification of RNA (Arvindhan Nagarajan, Radoslav Janostiak, Narendra Wajapeyee)....Pages 263-271
Mapping and Quantification of tRNA 2′-O-Methylation by RiboMethSeq (Adeline Galvanin, Lilia Ayadi, Mark Helm, Yuri Motorin, Virginie Marchand)....Pages 273-295
Back Matter ....Pages 297-298