دانلود کتاب Extracellular RNA: Methods and Protocols

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Front Matter ....Pages i-xi
Extracellular RNAs: A New Awareness of Old Perspectives (Noah Sadik, Lilian Cruz, Alessandra Gurtner, Rodosthenis S. Rodosthenous, Sophie A. Dusoswa, Olivia Ziegler et al.)....Pages 1-15
Overview of Protocols for Studying Extracellular RNA and Extracellular Vesicles (Julia Small, Sabrina Roy, Roger Alexander, Leonora Balaj)....Pages 17-21
Extracellular RNA Isolation from Cell Culture Supernatant (Sawen Bakr, Bridget Simonson, Kirsty M. Danielson, Saumya Das)....Pages 23-34
Use of a Hollow Fiber Bioreactor to Collect Extracellular Vesicles from Cells in Culture (Irene K. Yan, Neha Shukla, David A. Borrelli, Tushar Patel)....Pages 35-41
Isolation of Extracellular RNA from Serum/Plasma (Justyna Filant, Parham Nejad, Anu Paul, Bridget Simonson, Srimeenakshi Srinivasan, Xuan Zhang et al.)....Pages 43-57
Isolation of Extracellular RNA from Bile (Irene K. Yan, Valentin X. Berdah, Tushar Patel)....Pages 59-67
Cushioned–Density Gradient Ultracentrifugation (C-DGUC): A Refined and High Performance Method for the Isolation, Characterization, and Use of Exosomes (Kang Li, David K. Wong, King Yeung Hong, Robert L. Raffai)....Pages 69-83
Magnetic Particle-Based Immunoprecipitation of Nanoscale Extracellular Vesicles from Biofluids (Pete Heinzelman)....Pages 85-107
Enrichment of Extracellular Vesicle Subpopulations Via Affinity Chromatography (Michelle E. Hung, Stephen B. Lenzini, Devin M. Stranford, Joshua N. Leonard)....Pages 109-124
Detection and Analysis of Non-vesicular Extracellular RNA (Juan Pablo Tosar, Alfonso Cayota)....Pages 125-137
Isolation of Plasma Lipoproteins as a Source of Extracellular RNA (Kang Li, David K. Wong, Fu Sang Luk, Roy Y. Kim, Robert L. Raffai)....Pages 139-153
Droplet Digital PCR for Quantitation of Extracellular RNA (Irene K. Yan, Rishabh Lohray, Tushar Patel)....Pages 155-162
Preparation of Small RNA NGS Libraries from Biofluids (Alton Etheridge, Kai Wang, David Baxter, David Galas)....Pages 163-175
Multiplexed Detection and Quantitation of Extracellular Vesicle RNA Expression Using NanoString (Neha Shukla, Irene K. Yan, Tushar Patel)....Pages 177-185
Milk-derived Extracellular Vesicles for Therapeutic Delivery of Small Interfering RNAs (Akiko Matsuda, Tushar Patel)....Pages 187-197
Loading of Extracellular Vesicles with Hydrophobically Modified siRNAs (Marie-Cecile Didiot, Reka A. Haraszti, Neil Aronin, Anastasia Khvorova)....Pages 199-214
Back Matter ....Pages 215-216