دانلود کتاب FOXO Transcription Factors: Methods and Protocols

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Front Matter ....Pages i-xii
Introduction to FOXO Biology (Wolfgang Link)....Pages 1-9
Front Matter ....Pages 11-11
Characterization of MicroRNAs Regulating FOXO Expression (Doug Hanniford, Eva Hernando)....Pages 13-28
Identification of Transcription Factor-Binding Sites in the Mouse FOXO1 Promoter (Christopher P. Cardozo)....Pages 29-40
Investigating Mechanisms that Control Ubiquitin-Mediated DAF-16/FOXO Protein Turnover (Thomas Heimbucher, Coleen T. Murphy)....Pages 41-49
Phosphorylation of FOXO Proteins as a Key Mechanism to Regulate Their Activity (Alba Orea-Soufi, David Dávila, María Salazar-Roa, María de Mar Lorente, Guillermo Velasco)....Pages 51-59
Methodological Approach for the Evaluation of FOXO as a Positive Regulator of Antioxidant Genes (María Monsalve, Ignacio Prieto, Andreza Fabro de Bem, Yolanda Olmos)....Pages 61-76
Characterization of FOXO Acetylation (Shang Yao, Zimam Mahmud, Nikoleta Sachini, Sathid Aimjongjun, Paula Saavedra-García, Eric W-F Lam)....Pages 77-90
Transcriptional Activity of FOXO Transcription Factors Measured by Luciferase Assays (Antonia Ávila-Flores, Javier Arranz-Nicolás, Isabel Mérida)....Pages 91-102
Monitoring the Transcriptional Activity of FOXO Transcription Factors by Analyzing their Target Genes (Vanessa Henriques, Susana Machado, Wolfgang Link, Bibiana I. Ferreira)....Pages 103-113
Using ChIP-Based Approaches to Characterize FOXO Recruitment to its Target Promoters (Neeraj Kumar, Arnab Mukhopadhyay)....Pages 115-130
RNAi-Mediated Silencing of FOXO Factors (Priyankar Sanphui, Akash Saha, Subhas Chandra Biswas)....Pages 131-140
Front Matter ....Pages 141-141
Immunofluorescence Analysis by Confocal Microscopy for Detecting Endogenous FOXO (Francisco Castillo, Thomas A. Mackenzie, Bastien Cautain)....Pages 143-149
High-Throughput Image-Based Screening to Identify Chemical Compounds Capable of Activating FOXO (Marta Barradas, Wolfgang Link, Diego Megias, Pablo J. Fernandez-Marcos)....Pages 151-161
Image-based Identification of Chemical Compounds Capable of Trapping FOXO in the Cell Nucleus (Susana Machado, Catarina Raposo, Bibiana I. Ferreira, Wolfgang Link)....Pages 163-170
Quantifying Tissue-Specific Overexpression of FOXO in Drosophila via mRNA Fluorescence In Situ Hybridization Using Branched DNA Probe Technology (Anna C. Blice-Baum, Georg Vogler, Meera C. Viswanathan, Bosco Trinh, Worawan B. Limpitikul, Anthony Cammarato)....Pages 171-190
Front Matter ....Pages 191-191
Genome-Wide Analysis for Identifying FOXO Protein-Binding Sites (Dong-Ju Shin, Pujan Joshi, Dong-Guk Shin, Li Wang)....Pages 193-203
Mathematical Modeling of Nuclear Trafficking of FOXO Transcription Factors (Bradford E. Peercy, Martin F. Schneider)....Pages 205-217
Following Transcriptome to Uncover FOXO Biological Functions (Raymond Liang, Vijay Menon, Saghi Ghaffari)....Pages 219-227
Front Matter ....Pages 229-229
Hydra as Model to Determine the Role of FOXO in Longevity (Thomas C. G. Bosch)....Pages 231-238
Genetic Ablation of FOXO in Mice to Investigate Its Physiological Role (Hwanhee Oh, Jihye Paik)....Pages 239-248
Front Matter ....Pages 249-249
Analysis of FOXO3 Gene Polymorphisms Associated with Human Longevity (Timothy A. Donlon, Philip M. C. Davy, Bradley J. Willcox)....Pages 251-257
Analysis of FOXO3a Gene Polymorphism Associated with Asthma (Tejas Shah, Kalpana Joshi)....Pages 259-266
Back Matter ....Pages 267-270