توضیحاتی در مورد کتاب :
از پیشگفتار: چندین مقاله در این جلد به پیشرفتهای فناوری هیبریدوم میپردازد و مکمل فصل گالفری و میلشتاین است که در جلد 73 این مجموعه آمده است. روشهایی برای افزایش کارایی تولید محصولات همجوشی مورد نظر، سادهسازی روشهای اساسی درگیر، و ارزیابی ویژگی و سایر خواص آنتیبادیهای مونوکلونال مورد بحث قرار گرفتهاند. مثالهای نشاندهنده نشان میدهند که چگونه یک باتری از آنتیبادیهای مونوکلونال با ویژگیهای مختلف میتواند برای مطالعه مشکلات مختلف ایمونوشیمیایی و بیوشیمیایی استفاده شود. فصلهای دیگر روشهای دیگری را پوشش میدهند که میتوانند برای برچسبگذاری آنتیژنها و آنتیبادیها، جداسازی کمپلکسهای آنتیژن-آنتیبادی، و پردازش دادهها استفاده شوند. برخی از مقالاتی که تحت یک عنوان طبقه بندی می شوند یا در مجلد دیگری از این مجموعه قرار می گیرند، شامل روش هایی هستند که می توانند در زمینه های خاص دیگر اعمال شوند. برای مثال، در صورتی که فعالیت سیتوتوکسیک ویژگی مطلوب آنتی بادی مونوکلونال باشد، میتوان از آزمایشهای سمیت سلولی شرحدادهشده در جلد 93 برای غربالگری مایعات رویی در آزمایشهای هیبریدوم استفاده کرد.
فهرست مطالب :
Content:
Contributors to volume 92
Pages ix-xii
Preface
Page xiii
John J. Langone, Helen Van Vunakis
Volumes in series
Page xv
Volumes in preparation
Pages xvii-xxiv
[1] Human-human monoclonal antibody-producing hybridomas: Technical aspects Original Research Article
Pages 3-16
Lennart Olsson, Henry S. Kaplan
[2] Methods of enhancing the frequency of antigen-specific hybridomas Original Research Article
Pages 17-26
Reuben P. Siraganian, Philip C. Fox, Elsa H. Berenstein
[3] Spleen cell analysis and optimal immunization for high-frequency production of specific hybridomas Original Research Article
Pages 26-36
C. Stähli, Th. Staehelin, V. Miggiano
[4] Estimation of the number of monoclonal hybridomas in a cell-fusion experiment Original Research Article
Pages 36-39
Angel L. De Blas, Makarand V. Ratnaparkhi, James E. Mosimann
[5] Use of human endothelial culture supernatant (HECS) as a growth factor for hybridomas Original Research Article
Pages 39-47
Giulia C.B. Astaldi
[6] Production, purification, and characterization of antigen-specific murine monoclonal antibodies of IgE class Original Research Article
Pages 47-66
Amar S. Tung
[7] Production and properties of monoclonal antibodies to guinea pig Ia antigens Original Research Article
Pages 66-85
Stephen E. Zweig, Ethan M. Shevach
[8] Purification of murine MHC antigens by monoclonal antibody affinity chromatography Original Research Article
Pages 86-109
Matthew F. Mescher, Kathryn C. Stallcup, Cathleen P. Sullivan, Aaron P. Turkewitz, Steven H. Herrmann
[9] Monoclonal antibodies against HLA products and their use in immunoaffinity purification Original Research Article
Pages 110-138
Peter Parham
[10] Immunoassay for sequence-specific DNA-protein interaction Original Research Article
Pages 138-146
Ronald McKay
[11] Quantitation of hybridoma immunoglobulins and selection of light-chain loss variants Original Research Article
Pages 147-160
Timothy A. Springer
[12] Indirect 125I-labeled protein A assay for monoclonal antibodies to cell-surface antigens Original Research Article
Pages 160-168
Joseph P. Brown, Karl Erik Hellström, Ingegerd Hellström
[13] Enzyme-linked immunosorbent assay for screening monoclonal antibody production using enzyme-labeled second antibody Original Research Article
Pages 168-174
J.Y. Douillard, T. Hoffman
[14] Rosette-forming cell assay for detection of antibody-synthesizing hybridomas Original Research Article
Pages 175-182
Pierre Legrain, Dominique Juy, GГ©rard Buttin
[15] Rapid screening and replica plating of hybridomas for the production and characterization of monoclonal antibodies Original Research Article
Pages 182-195
Richard B. Bankert
[16] Use of high-resolution two-dimensional gel electrophoresis for analysis of monoclonal antibodies and their specific antigens Original Research Article
Pages 196-220
Terry W. Pearson, N. Leigh Anderson
[17] Screening of monoclonal immunoglobulins by immunofixation on cellulose acetate Original Research Article
Pages 220-227
M.A. Pizzolato
[18] Solid-phase immunofluorescence assay (SIFA) for detection and characterization of monoclonal antibodies against soluble antigens Original Research Article
Pages 227-237
Burkhard Micheel, Helmar Fiebach, Uwe Karsten
[19] Identification and characterization of lymphocyte hybridomas by electrophoresis of glucose-6-phosphate isomerase isozymes Original Research Article
Pages 237-242
Thomas J. Rogers, Kathleen O'Day
[20] Distinction of epitopes by monoclonal antibodies Original Research Article
Pages 242-253
C. Stähli, V. Miggiano, J. Stocker, Th. Staehelin, P. Häring, B. Takács
[21] Methods for the attachment of haptens and proteins to erythrocytes Original Research Article
Pages 257-276
Yi-Her Jou, Paul K. Mazzaferro, George L. Mayers, Richard B. Bankert
[22] Iodine monochloride (ICI) iodination techniques Original Research Article
Pages 277-292
M. Angeles Contreras, William F. Bale, Irving L. Spar
[23] Application of high-performance liquid chromatography to characterize radiolabeled peptides for radioimmunoassay, biosynthesis, and microsequence studies of polypeptide hormones Original Research Article
Pages 292-309
N.G. Seidah, M. ChrГ©tien
[24] Preparation of stable radioiodinated polypeptide hormones and proteins using polyacrylamide gel electrophoresis Original Research Article
Pages 309-335
Susanne Linde, Bruno Hansen, ГҐke Lernmark
[25] Noncentrifugation immunoassays: Novel systems Original Research Article
Pages 336-344
Michael Cais
[26] Use of activated thiol-sepharose in a separation method for enzyme immunoassay Original Research Article
Pages 345-359
Kanefusa Kato
[27] Affinity exclusion: A new method for the separation of free and bound fractions in enzyme immunoassay Original Research Article
Pages 359-366
B. Terouanne, J.C. Nicolas
[28] Use of chromatography tubes in the separation of bound and free fractions in radioimmunoassay Original Research Article
Pages 366-376
D.B. Wagner, J. Feingers, D. Inbar, A.J. Pick, Y. Tamir, O. Weiss
[29] Enzyme-linked immunoelectrotransfer blot techniques (EITB) for studying the specificities of antigens and antibodies separated by gel electrophoresis Original Research Article
Pages 377-391
Victor C.W. Tsang, Jose M. Peralta, A. Ray Simons
[30] Quantitative, single-tube, kinetic-dependent enzyme-linked immunosorbent assay (k-ELISA) Original Research Article
Pages 391-403
Victor C.W. Tsang, Britt C. Wilson, Jose M. Peralta
[31] Immunoassay using antigen-coated plastic tubes and radiolabeled or enzyme-labeled protein A Original Research Article
Pages 403-413
Adrian P. Gee, John J. Langone
[32] Colorimetric immunoassays using flavin adenine dinucleotide as label Original Research Article
Pages 413-425
David L. Morris, Robert T. Buckler
[33] Bioluminescent immunoassays Original Research Article
Pages 426-432
Jon Wannlund, Marlene DeLuca
[34] Immunoassay by electrochemical techniques Original Research Article
Pages 432-444
Kenneth R. Wehmeyer, Matthew J. Doyle, H. Brian Halsall, William R. Heineman
[35] Metalloimmunoassay: Principles and practice Original Research Article
Pages 445-458
Michael Cais
[36] Fluorescence fluctuation immunoassay Original Research Article
Pages 458-472
V.B. Elings, D.F. Nicoli, J. Briggs
[37] Methods and applications of hapten-sandwich labeling Original Research Article
Pages 472-488
Leon Wofsy
[38] Use of lectin-antibody conjugates for quantitation and titration of antigens and antibodies Original Research Article
Pages 489-498
Jean-Luc Guesdon, Stratis Avrameas
[39] Partition affinity ligand assay (PALA) for quantifying haptens, macromolecules, and whole cells Original Research Article
Pages 498-522
Bo Mattiasson
[40] Preparation and applications of multivalent antibodies with dual specificity Original Research Article
Pages 523-543
V. GhetЕЈie, I. Moraru
[41] LIGAND: A computerized analysis of ligand binding data Original Research Article
Pages 543-576
Peter J. Munson
[42] Two simple programs for the analysis of data from enzyme-linked immunosorbent assays (ELISA) on a programmable desk-top calculator Original Research Article
Pages 577-588
D.G. Ritchie, J.M. Nickerson, G.M. Fuller
[43] Determination of affinity and specificity of anti-hapten antibodies by competitive radioimmunoassay Original Research Article
Pages 589-601
Rolf MГјller
[44] Methods of measuring confidence limits in radioimmunoassay Original Research Article
Pages 601-610
Robert C. Baxter
Author index
Pages 611-627
Subject index
Pages 629-647
توضیحاتی در مورد کتاب به زبان اصلی :
FROM THE PREFACE: Several papers in this volume deal with advances in hybridoma technology and complement the chapter by Galfri and Milstein that appeared in Volume 73 of this series. Methods to enhance the efficiency of producing desired fusion products, to simplify the basic procedures involved, and to assess the specificity and other properties of monoclonal antibodies are discussed. Representative examples illustrate how a battery of monoclonal antibodies with different specificities can be used to study various immunochemical and biochemical problems. Other chapters cover additional procedures that can be used to label antigens and antibodies, separate antigen-antibody complexes, and process data. Some papers classified under one heading or contained in another volume of the series include methods that can be applied in other specific areas. For example, cytotoxicity tests described in Volume 93 may be used to screen supernatant fluids in hybridoma experiments if cytotoxic activity is a desirable property of the monoclonal antibody