دانلود کتاب Laboratory Methods in Enzymology: Protein Part C

فهرست مطالب :

Content:
Series Page
Page ii

Copyright
Page iv

Contributors
Pages xiii-xiv

Miscellaneous
Page xv

Preface
Page xvii
Jon Lorsch

Chapter One - TCA Precipitation
Pages 3-10
Laura Koontz

Chapter Two - Coimmunoprecipitation of Proteins from Yeast
Pages 13-26
Erica Gerace, Danesh Moazed

Chapter Three - Coupling Antibody to Cyanogen Bromide-Activated Sepharose
Pages 27-34
Jennifer M. Kavran, Daniel J. Leahy

Chapter Four - Analysis of Protein–Protein Interactions by Coimmunoprecipitation
Pages 35-47
Peter van der Geer

Chapter Five - Use and Application of Hydrophobic Interaction Chromatography for Protein Purification
Pages 51-65
Justin T. McCue

Chapter Six - Hydroxyapatite Chromatography: Purification Strategies for Recombinant Proteins
Pages 67-83
Larry J. Cummings

Chapter Seven - Salting out of Proteins Using Ammonium Sulfate Precipitation
Pages 85-94
Krisna C. Duong-Ly, Sandra B. Gabelli

Chapter Eight - Using Ion Exchange Chromatography to Purify a Recombinantly Expressed Protein
Pages 95-103
Krisna C. Duong-Ly, Sandra B. Gabelli

Chapter Nine - Gel Filtration Chromatography (Size Exclusion Chromatography) of Proteins
Pages 105-114
Krisna C. Duong-Ly, Sandra B. Gabelli

Chapter Ten - Expression and Purification of Membrane Proteins
Pages 117-140
Jan Kubicek, Helena Block, Barbara Maertens, Anne Spriestersbach, Jörg Labahn

Chapter Eleven - Explanatory Chapter: Choosing the Right Detergent
Pages 141-148
Dirk Linke

Chapter Twelve - One-dimensional SDS-Polyacrylamide Gel Electrophoresis (1D SDS-PAGE)
Pages 151-159
Julie L. Brunelle, Rachel Green

Chapter Thirteen - Coomassie Blue Staining
Pages 161-167
Julie L. Brunelle, Rachel Green

Chapter Fourteen - Silver Staining of SDS-polyacrylamide Gel
Pages 169-176
Jennifer M. Kavran, Daniel J. Leahy

Chapter Fifteen - Standard In Vitro Assays for Protein–Nucleic Acid Interactions – Gel Shift Assays for RNA and DNA Binding
Pages 179-196
Sarah F. Mitchell, Jon R. Lorsch

Chapter Sixteen - Protein Filter Binding
Pages 197-205
Sarah Kolitz, Jon R. Lorsch

Chapter Seventeen - Explanatory Chapter: Troubleshooting Recombinant Protein Expression: General
Pages 209-229
Krisna C. Duong-Ly, Sandra B. Gabelli

Chapter Eighteen - Explanatory Chapter: Troubleshooting Protein Expression: What to do When the Protein is not Soluble
Pages 231-247
Krisna C. Duong-Ly, Sandra B. Gabelli

Chapter Nineteen - Western Blotting using Chemiluminescent Substrates
Pages 251-259
Alice Alegria-Schaffer

Author Index
Pages 261-265

Subject Index
Pages 267-272