دانلود کتاب Manual of Research Techniques in Cardiovascular Medicine

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Content: Cover
Dedication
Title page
Copyright page
Contents
List of Contributors
Preface
About the Companion Website
Part 1: Electrophysiology
1: Measurement of calcium transient ex vivo
Introduction
Preparation and fluorescent indicator loading
Optical setup
Calibration
Analysis and interpretation
Set-by-step procedure
References
2: Confocal imaging of intracellular calcium cycling in isolated cardiac myocytes
Introduction
Materials and methods
Chamber, electrical stimulation, and superfusate delivery apparatus
Confocal microscope system. Cell loading of fluorescent Ca2+ indicatorsExperimental approach
Data analyses
Weaknesses and strengths of the method and alternative approaches
Conclusions
References
3: Generating a large animal model of persistent atrial fibrillation
Introduction
Insights from tachypacing-induced AF models
Protocol
Generating a tachypacing-induced AF model
Pacing protocols
Ventricular rate during AF: to ablate or not to ablate
Strengths and weaknesses of the model
Complications and alternative approaches to study AF
Potential complications
Alternative AF models
Conclusion
Acknowledgement. Choices to make before an experimentAP recording procedure
Frequent problems
AP analysis
Alternative approaches and extensions
Weaknesses and strengths
Conclusion
References
6: Patch-clamp recordings from isolated cardiac myocytes
Introduction
Theory of patch-clamp recording
Cell-attached configuration
Whole-cell recording configuration
Inside-out excised patch configuration
Outside-out excised patch configuration
Instrumentation for patch-clamp recordings
Experimental aspects of patch-clamp recordings
Gigaseal formation
Whole-cell recordings. Whole-cell voltage-clamp protocols, data acquisition, and analysisConclusions and future directions
References
7: Optical mapping of the heart
Introduction
Protocols
Experimental set-up
Tissue preparations (Figure 7.2)
Staining and image acquisition
Data processing and analysis
Representative results
Alternative approaches
Weaknesses and strengths of method
Conclusions
References
Part 2: Isolation and Maintenance of Primary Stem Cells
8: Isolation of colony-forming endothelial progenitor cells
Introduction
Materials
Laboratory reagents
Laboratory equipment
Methods.