دانلود کتاب Nuclear Reprogramming: Methods and Protocols

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Front Matter ....Pages i-xi
Pig Cloning Using Somatic Cell Nuclear Transfer (Hongsheng Ouyang, Jianyong Han, Yongye Huang)....Pages 1-18
Cloning of Monkeys by Somatic Cell Nuclear Transfer (Shiyu Sun, Zhaodi Liao, Zhen Liu, Qiang Sun)....Pages 19-31
Production of Cardiomyocyte-Like Cells by Fibroblast Reprogramming with Defined Factors (Emre Bektik, Ji-Dong Fu)....Pages 33-46
Production of Cardiomyocytes by microRNA-Mediated Reprogramming in Optimized Reprogramming Media (Xiaowen Wang, Conrad P. Hodgkinson, Victor J. Dzau)....Pages 47-59
Direct Reprogramming of Human Fibroblasts into Induced Neural Progenitor Cells Using Suicide Gene Embodied Episomal Vectors for Rapid Selection of Exogenous DNA-Free Cells (Minhyung Lee, Janghwan Kim, Rajesh Ambasudhan)....Pages 61-75
Generation of Human Neurons by microRNA-Mediated Direct Conversion of Dermal Fibroblasts (Victoria A. Church, Kitra Cates, Lucia Capano, Shivani Aryal, Woo Kyung Kim, Andrew S. Yoo)....Pages 77-100
Reprogramming Human Fibroblasts to Induced Pluripotent Stem Cells Using the GFP-Marked Lentiviral Vectors in the Chemically Defined Medium (Zhicheng Shao, Ricardo Raúl Cevallos, Kejin Hu)....Pages 101-116
Episomal Reprogramming of Human Peripheral Blood Mononuclear Cells into Pluripotency (Wei Wen, Tao Cheng, Xiao-Bing Zhang)....Pages 117-133
Generation of Human iPSCs by Episomal Reprogramming of Skin Fibroblasts and Peripheral Blood Mononuclear Cells (Fabia Febbraro, Muwan Chen, Mark Denham)....Pages 135-151
Generation of Human iPSCs by Protein Reprogramming and Stimulation of TLR3 Signaling (Chun Liu, Mohamed Ameen, Sukaina Himmati, Dilip Thomas, Nazish Sayed)....Pages 153-162
Generation of Human iPSCs by Reprogramming with the Unmodified Synthetic mRNA (Robert R. Annand)....Pages 163-174
Reprogramming of Fibroblasts to Human iPSCs by CRISPR Activators (Jere Weltner, Ras Trokovic)....Pages 175-198
Reprogramming Porcine Fibroblast to EPSCs (Xuefei Gao, Degong Ruan, Pentao Liu)....Pages 199-211
Preparation of Mouse Embryonic Fibroblasts as Feeders for iPSC Generation and Maintenance (Jennifer L. Brehm, Molly M. Miles, Katie S. Remondini, Tenneille E. Ludwig)....Pages 213-234
Evaluating Reprogramming Efficiency and Pluripotency of the Established Human iPSCS by Pluripotency Markers (Ricardo Raúl Cevallos, Md Emon Hossain, Ruowen Zhang, Kejin Hu)....Pages 235-249
G-Banded Karyotyping of Human Pluripotent Stem Cell Cultures (Erik McIntire, Kimberly Leonhard, Seth Taapken, Anna Lisa Larson)....Pages 251-268
Become Competent in Generating RNA-Seq Heat Maps in One Day for Novices Without Prior R Experience (Kejin Hu)....Pages 269-303
Back Matter ....Pages 305-306