دانلود کتاب Proteomics for Biomarker Discovery: Methods and Protocols

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Front Matter ....Pages i-xii
Pre- and Post-analytical Factors in Biomarker Discovery (Frank Klont, Peter Horvatovich, Natalia Govorukhina, Rainer Bischoff)....Pages 1-22
Pre-fractionation of Noncirculating Biological Fluids to Improve Discovery of Clinically Relevant Protein Biomarkers (Annarita Farina)....Pages 23-37
Serum Exosome Isolation by Size-Exclusion Chromatography for the Discovery and Validation of Preeclampsia-Associated Biomarkers (Rosana Navajas, Fernando J. Corrales, Alberto Paradela)....Pages 39-50
Protein Biomarker Discovery Using Human Blood Plasma Microparticles (Raghda Saad Zaghloul Taleb, Pacint Moez, Doreen Younan, Martin Eisenacher, Matthias Tenbusch, Barbara Sitek et al.)....Pages 51-64
A Standardized and Reproducible Proteomics Protocol for Bottom-Up Quantitative Analysis of Protein Samples Using SP3 and Mass Spectrometry (Christopher S. Hughes, Poul H. Sorensen, Gregg B. Morin)....Pages 65-87
Analyzing Cerebrospinal Fluid Proteomes to Characterize Central Nervous System Disorders: A Highly Automated Mass Spectrometry-Based Pipeline for Biomarker Discovery (Antonio Núñez Galindo, Charlotte Macron, Ornella Cominetti, Loïc Dayon)....Pages 89-112
Lys-C/Trypsin Tandem-Digestion Protocol for Gel-Free Proteomic Analysis of Colon Biopsies (Armin Schniers, Yvonne Pasing, Terkel Hansen)....Pages 113-122
Tube-Gel: A Fast and Effective Sample Preparation Method for High-Throughput Quantitative Proteomics (Leslie Muller, Luc Fornecker, Sarah Cianferani, Christine Carapito)....Pages 123-127
Protein Biomarker Discovery in Non-depleted Serum by Spectral Library-Based Data-Independent Acquisition Mass Spectrometry (Alexandra Kraut, Mathilde Louwagie, Christophe Bruley, Christophe Masselon, Yohann Couté, Virginie Brun et al.)....Pages 129-150
Discovering Protein Biomarkers from Clinical Peripheral Blood Mononuclear Cells Using Data-Independent Acquisition Mass Spectrometry (Xin Ku, Wei Yan)....Pages 151-161
Intact Protein Analysis by LC-MS for Characterizing Biomarkers in Cerebrospinal Fluid (Jérôme Vialaret, Sylvain Lehmann, Christophe Hirtz)....Pages 163-172
Detection of Proteoforms Using Top-Down Mass Spectrometry and Diagnostic Ions (Didia Coelho Graça, Ralf Hartmer, Wolfgang Jabs, Alexander Scherl, Lorella Clerici, Kaveh Samii et al.)....Pages 173-183
Development of a Highly Multiplexed SRM Assay for Biomarker Discovery in Formalin-Fixed Paraffin-Embedded Tissues (Carine Steiner, Pierre Lescuyer, Jean-Christophe Tille, Paul Cutler, Axel Ducret)....Pages 185-203
Development and Validation of Multiple Reaction Monitoring (MRM) Assays for Clinical Applications (Georgia Kontostathi, Manousos Makridakis, Vasiliki Bitsika, Nikolaos Tsolakos, Antonia Vlahou, Jerome Zoidakis)....Pages 205-223
Protein-Level Statistical Analysis of Quantitative Label-Free Proteomics Data with ProStaR (Samuel Wieczorek, Florence Combes, Hélène Borges, Thomas Burger)....Pages 225-246
Computation and Selection of Optimal Biomarker Combinations by Integrative ROC Analysis Using CombiROC (Mauro Bombaci, Riccardo L. Rossi)....Pages 247-259
PanelomiX for the Combination of Biomarkers (Xavier Robin)....Pages 261-273
Designing an In Silico Strategy to Select Tissue-Leakage Biomarkers Using the Galaxy Framework (Lien Nguyen, Virginie Brun, Florence Combes, Valentin Loux, Yves Vandenbrouck)....Pages 275-289
Back Matter ....Pages 291-292