دانلود کتاب RNA Spectroscopy: Methods and Protocols

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Front Matter ....Pages i-xi
Encapsulation of Fluorescently Labeled RNAs into Surface-Tethered Vesicles for Single-Molecule FRET Studies in TIRF Microscopy (Susann Zelger-Paulus, Mélodie C. A. S. Hadzic, Roland K. O. Sigel, Richard Börner)....Pages 1-16
Preparation of SNAPf-Beads for Colocalization Single-Molecule Spectroscopy (CoSMoS) of RNA-Protein Complexes (Reka A. Haraszti, Joerg E. Braun)....Pages 17-22
Comparative Colocalization Single-Molecule Spectroscopy (CoSMoS) with Multiple RNA Species (Reka A. Haraszti, Joerg E. Braun)....Pages 23-29
Dynamic Light Scattering Analysis on RNA Associated to Proteins (Serena Bernacchi)....Pages 31-39
Bioinformatic Application of Fluorescence-Based In Vivo RNA Regional Accessibility Data to Identify Novel sRNA Targets (Emily K. Bowman, Mia K. Mihailovic, Bridget Li, Lydia M. Contreras)....Pages 41-71
Surface Plasmon Resonance for Investigating Molecular Interactions with RNA (Carmelo Di Primo)....Pages 73-88
RNA Folding and Unfolding Under Force: Single-Molecule Experiments and Their Analysis (Laurent Geffroy, Thierry Bizebard, Ulrich Bockelmann)....Pages 89-100
Mapping of Posttranscriptional tRNA Modifications by Two-Dimensional Gel Electrophoresis Mass Spectrometry (Laura Antoine, Philippe Wolff)....Pages 101-110
Native Electrospray Ionization Mass Spectrometry of RNA-Ligand Complexes (Philippe Wolff, Eric Ennifar)....Pages 111-118
Application of FTIR Spectroscopy to Analyze RNA Structure (Frédéric Geinguenaud, Valeria Militello, Véronique Arluison)....Pages 119-133
Application of Synchrotron Radiation Circular Dichroism for RNA Structural Analysis (Etienne Le Brun, Véronique Arluison, Frank Wien)....Pages 135-148
Application of NIR Raman Spectroscopy to Probe the Flexibility of RNA Structure (Hui Bon Hoa Gaston)....Pages 149-164
Small-Angle Neutron Scattering of RNA–Protein Complexes (Audrone Lapinaite, Teresa Carlomagno, Frank Gabel)....Pages 165-188
Structural Analysis of RNA by Small-Angle X-ray Scattering (Anne Théobald-Dietrich, Raphaël de Wijn, Kévin Rollet, Alexandra Bluhm, Joëlle Rudinger-Thirion, Caroline Paulus et al.)....Pages 189-215
Site-Specific Spin Labeling of RNA for NMR and EPR Structural Studies (Bertrand Vileno, Isabelle Lebars)....Pages 217-235
Analysis of the HIV-1 Genomic RNA Dimerization Initiation Site Binding to Aminoglycoside Antibiotics Using Isothermal Titration Calorimetry (Serena Bernacchi, Eric Ennifar)....Pages 237-250
Use of Fluorescent Nucleotides to Map RNA-Binding Sites on Protein Surface (V. Balobanov, N. Lekontseva, A. Mikhaylina, A. Nikulin)....Pages 251-262
Fluorescent Oligonucleotide Probes for the Quantification of RNA by Real-Time qPCR (Florent Busi)....Pages 263-280
Probing RNA–Protein Interactions and RNA Compaction by Sedimentation Velocity Analytical Ultracentrifugation (Somdeb Mitra, Borries Demeler)....Pages 281-317
RNA Nanostructure Molecular Imaging (Olivier Piétrement, Véronique Arluison, Christophe Lavelle)....Pages 319-327
Grad-cryo-EM: Tool to Isolate Translation Initiation Complexes from Rabbit Reticulocyte Lysate Suitable for Structural Studies (Javier Rol-Moreno, Lauriane Kuhn, Stefano Marzi, Angelita Simonetti)....Pages 329-339
Application of NMR Spectroscopy to Determine Small RNA Structure (Pengzhi Wu, Xiaodan Liu, Qingguo Gong, Yunyu Shi, Jihui Wu)....Pages 341-353
Back Matter ....Pages 355-356