دانلود کتاب T-Cell Motility: Methods and Protocols

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Front Matter ....Pages i-x
An Introduction to LFA-1/ICAM-1 Interactions in T-Cell Motility (Navin Kumar Verma, Dermot Kelleher)....Pages 1-9
Isolation of Human Peripheral Blood T-Lymphocytes (Atish Kizhakeyil, Seow Theng Ong, Mobashar Hussain Urf Turabe Fazil, Madhavi Latha Somaraju Chalasani, Praseetha Prasannan, Navin Kumar Verma)....Pages 11-17
A Laboratory Model to Study T-Cell Motility (Navin Kumar Verma, Shyam Mohan Palapetta, Seow Theng Ong, Mobashar Hussain Urf Turabe Fazil, Madhavi Latha Somaraju Chalasani, Praseetha Prasannan et al.)....Pages 19-23
Quantification of T-Cell Migratory Phenotypes Using High-Content Analysis (Aik Seng Ng, Seow Theng Ong, Dermot Kelleher, Navin Kumar Verma)....Pages 25-32
Live Cell Imaging and Analysis to Capture T-Cell Motility in Real-Time (Seow Theng Ong, Navin Kumar Verma)....Pages 33-40
Three-Dimensional Structured Illumination Microscopy (3D-SIM) to Dissect Signaling Cross-Talks in Motile T-Cells (Seow Theng Ong, Graham D. Wright, Navin Kumar Verma)....Pages 41-50
Real-Time Impedance-Based Detection of LFA-1-Stimulated T-Cell Transwell Chemotaxis (Praseetha Prasannan, Navin Kumar Verma)....Pages 51-57
Quantitative Real-Time PCR for Evaluating Transcriptional Changes in T-Lymphocytes (Atish Kizhakeyil, Navin Kumar Verma)....Pages 59-66
GapmeR-Mediated Gene Silencing in Motile T-Cells (Mobashar Hussain Urf Turabe Fazil, Seow Theng Ong, Madhavi Latha Somaraju Chalasani, Atish Kizhakeyil, Navin Kumar Verma)....Pages 67-73
Live Imaging of Resident T-Cell Migration in Human Lymphoid Tissue Slices Using Confocal Microscopy (Emmanuel Donnadieu, Yvonne Michel, Martin-Leo Hansmann)....Pages 75-82
Phosphoprotein Enrichment for Protein Analysis in Motile T-Lymphocytes (Praseetha Prasannan, Rathi Saravanan, Navin Kumar Verma)....Pages 83-90
Immunometabolomic Phenotyping of Motile T-Cells (Madhavi Latha Somaraju Chalasani, Gaelin Looi Wen Dong, Navin Kumar Verma)....Pages 91-98
Profiling Activity of Cellular Kinases in Migrating T-Cells (Chandra Sekhar Chirumamilla, Mobashar Hussain Urf Turabe Fazil, Claudina Perez-Novo, Savithri Rangarajan, Rik de Wijn, Padma Ramireddy et al.)....Pages 99-113
Biotinylation Assay to Determine LFA-1 Recycling in Motile T-Lymphocytes (Malin Samuelsson, Lena M. Svensson)....Pages 115-122
A Protocol to Study T-Cell Signaling in an Immune Synapse by Microscopy (Mobashar Hussain Urf Turabe Fazil, Pankaj Kumar, Navin Kumar Verma)....Pages 123-128
Enzyme-Linked Immunosorbent Assay for T-Cell Dependent Immunogenicity Assessment of Therapeutic Peptides (Madhavi Latha Somaraju Chalasani, Rajamani Lakshminarayanan, Navin Kumar Verma)....Pages 129-138
Modified Intravital Microscopy to Assess Vascular Health and T-Cell Motility (Geoffrey W. Payne, Kevin Mitchell, Stephanie L. Sellers)....Pages 139-147
Computational Analysis of Protein–Protein Interactions in Motile T-Cells (Sunil Kumar, Mobashar Hussain Urf Turabe Fazil, Khurshid Ahmad, Minaketan Tripathy, Jagath Chandana Rajapakse, Navin Kumar Verma)....Pages 149-156
Back Matter ....Pages 157-158