دانلود کتاب The CRAC Channel: Methods and Protocols

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Front Matter ....Pages i-xi
Patch-Clamp Recording of the CRAC Channel Current in STIM-Orai Overexpressing Cells (Yiming Zhang, Sisi Zheng, Yubin Zhou, Donald L. Gill, Youjun Wang)....Pages 1-16
Fluorescence-Based Ratiometric Measurement of CRAC Channel Activity in STIM-Orai-Overexpressing HEK-293 Cells (Shuce Zhang, Lian He, Yubin Zhou, Youjun Wang)....Pages 17-39
Recording SOCE Activity in Neurons by Patch-Clamp Electrophysiology and Microfluorometric Calcium Imaging (Hsiang-en Wu, Geza Gemes, Quinn H. Hogan)....Pages 41-53
Mn2+ Quenching Assay for Store-Operated Calcium Entry (Zui Pan, Sangyong Choi, Yanhong Luo)....Pages 55-62
Fluorescence-Based Measurements of Store-Operated Ca2+ Entry in Cancer Cells Using Fluo-4 and Confocal Live-Cell Imaging (Fujian Lu, Jianwei Sun, Tao Sun, Heping Cheng, Shengyu Yang)....Pages 63-68
Fluorescence-Based Measurements of the CRAC Channel Activity in Cell Populations (Pedro C. Redondo, Alejandro Berna-Erro, Natalia Dionisio, Juan A. Rosado)....Pages 69-82
Indirect Measurement of CRAC Channel Activity Using NFAT Nuclear Translocation by Flow Cytometry in Jurkat Cells (María D. Carretta, María A. Hidalgo, Rafael A. Burgos)....Pages 83-94
CRAC Channel Components Quantitative Expression (In Tissues and Cell Lines) Using qPCR (Charlotte Dubois, Vyacheslav Lehenkyi, Natalia Prevarskaya)....Pages 95-106
Western Blotting and Co-immunoprecipitation of Endogenous STIM/ORAI and Protein Partners (Charlotte Dubois, Natalia Prevarskaya)....Pages 107-113
Study of Endogenous CRAC Channels in Human Mast Cells Using an Adenoviral Delivery System to Transduce Cells with Orai-Targeting shRNAs or with cDNAs Expressing Dominant-Negative Orai Channel Mutations (Ian Ashmole, Peter Bradding)....Pages 115-124
Store-Operated Ca2+ Entry in Drosophila Primary Neuronal Cultures (Sumita Chakraborty, Gaiti Hasan)....Pages 125-136
Study of the Endogenous CRAC Channel Using shRNA-Mediated Gene Silencing (Xuexin Zhang, Amy M. Spinelli, Wei Zhang, Mohamed Trebak)....Pages 137-145
Engineered Cross-Linking to Study the Pore Architecture of the CRAC Channel (Guolin Ma, Lian He, Ji Jing, Peng Tan, Yun Huang, Yubin Zhou)....Pages 147-166
Measurement of the CRAC Channel Fast Ca2+-Dependent Inactivation (FCDI) (Grigori Y. Rychkov)....Pages 167-173
High-Resolution Imaging of STIM/Orai Subcellular Localization Using Array Confocal Laser Scanning Microscopy (Andras T. Deak, Benjamin Gottschalk, Emrah Eroglu, Rene Rost, Markus Waldeck-Weiermair, Wolfgang F. Graier et al.)....Pages 175-187
Single-Channel Single-Molecule Detection (SC-SMD) System (Laura G. Ceballos, Alexander Asanov, Luis Vaca)....Pages 189-201
Back Matter ....Pages 203-204