دانلود کتاب Transgenic Mouse: Methods and Protocols

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Front Matter ....Pages i-x
The History of Transgenesis (Thomas L. Saunders)....Pages 1-26
Pronuclear Microinjection of One-Cell Embryos (Melissa A. Larson)....Pages 27-33
Integrase-Mediated Targeted Transgenics Through Pronuclear Microinjection (Ruby Yanru Chen-Tsai)....Pages 35-46
In Vivo Validation of CRISPR Reagents in Preimplantation Mouse Embryos (Melissa A. Larson, Katelin A. Gibson, Jay L. Vivian)....Pages 47-57
Targeted Mutations in the Mouse via Embryonic Stem Cells (Marina Gertsenstein, Joffrey Mianné, Lydia Teboul, Lauryl M. J. Nutter)....Pages 59-82
Blastocyst Microinjection with Embryonic Stem Cells (Melissa A. Larson)....Pages 83-88
Generation of Large Fragment Knock-In Mouse Models by Microinjecting into 2-Cell Stage Embryos (Bin Gu, Marina Gertsenstein, Eszter Posfai)....Pages 89-100
Embryo Transfer Surgery (Melissa A. Larson)....Pages 101-106
Nonsurgical Embryo Transfer Protocol for Use with the NSET™ Device (Barbara J. Stone)....Pages 107-111
Nuclear Transfer and Cloning (Ling Liu)....Pages 113-124
Sperm-Mediated Genetic Modifications (Marialuisa Lavitrano, Laura Farina, Maria Grazia Cerrito, Roberto Giovannoni)....Pages 125-132
Genotyping Genetically Modified (GM) Mice (Neeraj K. Aryal, Jan Parker-Thornburg)....Pages 133-148
Nomenclature: Naming Your Gene-Modified Mouse (Alicia Valenzuela)....Pages 149-162
Breeding Strategies for Genetically Modified Mice (Jan Parker-Thornburg)....Pages 163-169
Strategies for Behaviorally Phenotyping the Transgenic Mouse (Kenneth E. McCarson)....Pages 171-194
Cryobanking and Recovery of Genetically Modified Mice (Toru Takeo, Naomi Nakagata)....Pages 195-209
Simple Transportation of Genetically Engineered Mice via Cold Storage Techniques (Hidetaka Yoshimoto, Toru Takeo, Naomi Nakagata)....Pages 211-216
Reprogramming of Primary Human Cells to Induced Pluripotent Stem Cells Using Sendai Virus (Julia M. Draper, Jay L. Vivian)....Pages 217-234
Back Matter ....Pages 235-236