دانلود کتاب Trinucleotide Repeats: Methods and Protocols

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Front Matter ....Pages i-xi
Experimenting with Trinucleotide Repeats: Facts and Technical Issues (Guy-Franck Richard)....Pages 1-10
Fast Assays to Detect Interruptions in CTG.CAG Repeat Expansions (Stéphanie Tomé, Geneviève Gourdon)....Pages 11-23
Tracking Expansions of Stable and Threshold Length Trinucleotide Repeat Tracts In Vivo and In Vitro Using Saccharomyces cerevisiae (Gregory M. Williams, Athena K. Petrides, Lata Balakrishnan, Jennifer A. Surtees)....Pages 25-68
Quantifying Replication Fork Progression at CTG Repeats by 2D Gel Electrophoresis (David Viterbo, Guy-Franck Richard)....Pages 69-81
Genetic Assays to Study Repeat Fragility in Saccharomyces cerevisiae (Erica J. Polleys, Catherine H. Freudenreich)....Pages 83-101
Genetic Screens to Study GAA/TTC and Inverted Repeat Instability in Saccharomyces cerevisiae (Wenying Guo, Kirill S. Lobachev)....Pages 103-112
Monitoring Double-Strand Break Repair of Trinucleotide Repeats Using a Yeast Fluorescent Reporter Assay (Lucie Poggi, Bruno Dumas, Guy-Franck Richard)....Pages 113-120
Analysis of Trinucleotide Repeat Stability by Integration at a Chromosomal Ectopic Site (Rujuta Yashodhan Gadgil, S. Dean Rider Jr., Todd Lewis, Joanna Barthelemy, Michael Leffak)....Pages 121-136
Experimental System to Study Instability of (CGG)n Repeats in Cultured Mammalian Cells (Artem V. Kononenko, Thomas Ebersole, Sergei M. Mirkin)....Pages 137-150
Assessing Triplet Repeat Expansions in Human SVG-A Cell Culture (Gregory M. Williams, Robert S. Lahue)....Pages 151-172
Isolation and Analysis of the CGG-Repeat Size in Male and Female Gametes from a Fragile X Mouse Model (Xiaonan Zhao, Huiyan Lu, Pradeep K. Dagur, Karen Usdin)....Pages 173-186
In Vitro Synthesis and RNA Structure Probing of CUG Triplet Repeat RNA (Remco T. P. van Cruchten, Derick G. Wansink)....Pages 187-202
FISH Protocol for Myotonic Dystrophy Type 1 Cells (Arnaud F. Klein, Ludovic Arandel, Joelle Marie, Denis Furling)....Pages 203-215
Real Time Videomicroscopy and Semiautomated Analysis of Brain Cell Culture Models of Trinucleotide Repeat Expansion Diseases (Sandra O. Braz, Diana M. Dinca, Geneviève Gourdon, Mário Gomes-Pereira)....Pages 217-240
Primary Cultures of Pure Embryonic Dorsal Root Ganglia Sensory Neurons as a New Cellular Model for Friedreich’s Ataxia (Olivier Griso, Hélène Puccio)....Pages 241-253
GFP Reporters to Monitor Instability and Expression of Expanded CAG/CTG Repeats (Cinzia Cinesi, Bin Yang, Vincent Dion)....Pages 255-268
Gene Therapy for Huntington’s Disease Using Targeted Endonucleases (Magdalena Dabrowska, Marta Olejniczak)....Pages 269-284
Back Matter ....Pages 285-286